Hotspot

trnL-CAA-ycf2

IRa · 149192-149530 · 339 bp

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Pi 0.0442
MarkerSeek score 63.90
Primer available yes
Label trnL-CAA-ycf2

Feature Metadata

label_name
trnL-CAA-ycf2
feature_type
igs
region
IRa
length_bp
339
variable_sites
15
pi
0.0442
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-CAA-ycf2_p1 AAATGGTAGACACGCGAGAC CAGCCCTTTGTCTCTGTGTT 50.0 50.0 58.0 58.0 762 bp 1.0 81.9
trnL-CAA-ycf2_p2 TGAAATGGTAGACACGCGAG CAGCCCTTTGTCTCTGTGTT 50.0 50.0 58.0 58.0 764 bp 1.0 81.9
trnL-CAA-ycf2_p3 AAATGGTAGACACGCGAGAC GTTCTTGCAGAGTGGAACCA 50.0 50.0 58.0 58.0 886 bp 1.0 81.6
trnL-CAA-ycf2_p4 TGAAATGGTAGACACGCGAG GTTCTTGCAGAGTGGAACCA 50.0 50.0 58.0 58.0 888 bp 1.0 81.6
trnL-CAA-ycf2_p5 AAATGGTAGACACGCGAGAC TATTGGGCCAGGTCATTTCG 50.0 50.0 58.0 57.9 960 bp 1.0 81.3