Hotspot

trnL-UAG-trnI-CAU

SSC · 118725-118767 · 43 bp

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Pi 0.0233
MarkerSeek score 61.70
Primer available yes
Label trnL-UAG-trnI-CAU

Feature Metadata

label_name
trnL-UAG-trnI-CAU
feature_type
igs
region
SSC
length_bp
43
variable_sites
1
pi
0.0233
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAG-trnI-CAU_p1 TTATTTCGATGAAGCCGCCA TAGAATTTGGGTCACTCGCG 45.0 50.0 58.0 58.0 251 bp 1.0 80.0
trnL-UAG-trnI-CAU_p2 TTATTTCGATGAAGCCGCCA ACTCGCGATGTACAATGACC 45.0 50.0 58.0 58.1 238 bp 1.0 79.5
trnL-UAG-trnI-CAU_p3 TTATTTCGATGAAGCCGCCA GGTCACTCGCGATGTACAAT 45.0 50.0 58.0 58.1 242 bp 1.0 79.5
trnL-UAG-trnI-CAU_p4 TAGACACGCTGCTCTTAGGA TAGAATTTGGGTCACTCGCG 50.0 50.0 57.9 58.0 219 bp 1.0 79.3
trnL-UAG-trnI-CAU_p5 TTATTTCGATGAAGCCGCCA AATGACCCCAGTGAAGCATC 45.0 50.0 58.0 57.9 225 bp 1.0 79.0