Hotspot

petA-psbJ

LSC · 66178-67319 · 1142 bp

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Pi 0.0123
MarkerSeek score 65.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1142
variable_sites
14
pi
0.0123
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 ACGTGTCCAAGGTTTGTTGT GGCTCATATTCCGGATTGGG 45.0 55.0 58.2 58.2 1287-1288 bp 1.0 78.1
petA-psbJ_p2 ACGTGTCCAAGGTTTGTTGT CGGCTCATATTCCGGATTGG 45.0 55.0 58.2 58.6 1288-1289 bp 1.0 75.0
petA-psbJ_p3 ACGTGTCCAAGGTTTGTTGT GCTCATATTCCGGATTGGGA 45.0 50.0 58.2 57.1 1286-1287 bp 1.0 72.6
petA-psbJ_p4 ACGTGTCCAAGGTTTGTTGT ATTCCGGATTGGGATCATCC 45.0 50.0 58.2 57.1 1280-1281 bp 1.0 72.4
petA-psbJ_p5 CGTGTCCAAGGTTTGTTGTT GGCTCATATTCCGGATTGGG 45.0 55.0 57.1 58.2 1286-1287 bp 1.0 72.0