Hotspot

petN-psbM

LSC · 29968-31030 · 1063 bp

Back to Aiouea

Pi 0.00188
MarkerSeek score 59.80
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1063
variable_sites
2
pi
0.00188
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TAGTAAGTCTCGCTTGGGCT TGCTACTGCGTTGTTCATTCT 50.0 42.9 58.2 58.2 1225 bp 1.0 69.1
petN-psbM_p2 TAGTAAGTCTCGCTTGGGCT TGCTACTGCGTTGTTCATTC 50.0 45.0 58.2 56.7 1225 bp 1.0 68.9
petN-psbM_p3 GGGACACGATTCACATGGAT TGCTACTGCGTTGTTCATTCT 50.0 42.9 57.7 58.2 1246 bp 1.0 67.6
petN-psbM_p4 GGGACACGATTCACATGGAT TGCTACTGCGTTGTTCATTC 50.0 45.0 57.7 56.7 1246 bp 1.0 67.5
petN-psbM_p5 ATGGGGAAGAAGTGGACTCT TGCTACTGCGTTGTTCATTCT 50.0 42.9 57.6 58.2 1164 bp 1.0 67.5