Hotspot

rpl16

LSC · 81093-82489 · 1397 bp

Back to Ainsliaea

Pi 0.00143

MarkerSeek score 59.60

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1397
variable_sites: 2
pi: 0.00143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAAAGAAAACTGCACCCACT	TCGTTTCTTCTTTTTCTTTTCTGT	40.0	29.2	56.0	55.7	1556-1557 bp	1.0	40.1
rpl16_p2	AAAAGAAAACTGCACCCACT	CGTTTCTTCTTTTTCTTTTCTGTTCA	40.0	30.8	56.0	57.6	1555-1556 bp	1.0	40.1
rpl16_p3	AAAAAGAAAACTGCACCCACT	TCGTTTCTTCTTTTTCTTTTCTGT	38.1	29.2	56.7	55.7	1557-1558 bp	1.0	40.1
rpl16_p4	AAAAGAAAACTGCACCCACT	TCGTTTCTTCTTTTTCTTTTCTGTT	40.0	28.0	56.0	56.4	1556-1557 bp	1.0	40.1
rpl16_p5	AAAAGAAAACTGCACCCACT	TTCGTTTCTTCTTTTTCTTTTCTGT	40.0	28.0	56.0	56.4	1557-1558 bp	1.0	40.1