Hotspot

ndhI

SSC · 118484-118984 · 501 bp

Back to Ainsliaea

Pi 0.002

MarkerSeek score 57.80

Primer available yes

Label ndhI

Feature Metadata

label_name: ndhI
feature_type: gene
region: SSC
length_bp: 501
variable_sites: 1
pi: 0.002
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhI_p1	TGAAAACACGATTCTCGCCT	ACTTCTTCCCAACTTCTTTCACT	45.0	39.1	57.8	57.9	710 bp	1.0	53.9
ndhI_p2	AACACGATTCTCGCCTCCTA	ACTTCTTCCCAACTTCTTTCACT	50.0	39.1	58.5	57.9	706 bp	1.0	50.9
ndhI_p3	ACACGATTCTCGCCTCCTAA	ACTTCTTCCCAACTTCTTTCACT	50.0	39.1	58.5	57.9	705 bp	1.0	50.9
ndhI_p4	AAAACACGATTCTCGCCTCC	ACTTCTTCCCAACTTCTTTCACT	50.0	39.1	58.6	57.9	708 bp	1.0	50.7
ndhI_p5	TGAAAACACGATTCTCGCCT	ACTTCTTCCCAACTTCTTTCAC	45.0	40.9	57.8	56.5	710 bp	1.0	50.6