Hotspot

ndhC

LSC · 49975-50337 · 363 bp

Back to Ainsliaea

Pi 0

MarkerSeek score 35.10

Primer available yes

Label ndhC

Feature Metadata

label_name: ndhC
feature_type: gene
region: LSC
length_bp: 363
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC_p1	GGGTTGTTCGATCAAATAAGGG	CCACGTTCATAGGAGTGCAT	45.5	50.0	57.4	57.7	476 bp	1.0	56.4
ndhC_p2	GGGTTGTTCGATCAAATAAGGG	TTCCACGTTCATAGGAGTGC	45.5	50.0	57.4	57.6	478 bp	1.0	55.9
ndhC_p3	ACTGAATTTTGGGTTGTTCGA	CCACGTTCATAGGAGTGCAT	38.1	50.0	56.3	57.7	486 bp	1.0	55.7
ndhC_p4	ACTGAATTTTGGGTTGTTCGA	TTCCACGTTCATAGGAGTGC	38.1	50.0	56.3	57.6	488 bp	1.0	55.2
ndhC_p5	AACTGAATTTTGGGTTGTTCGA	CCACGTTCATAGGAGTGCAT	36.4	50.0	57.0	57.7	487 bp	1.0	53.5