Hotspot

cemA

LSC · 61137-61826 · 690 bp

Back to Ainsliaea

Pi 0.00145

MarkerSeek score 59.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 690
variable_sites: 1
pi: 0.00145
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	GAGTCGACGAATGAGATGGG	GCTTTGCTTATGTACAACTGCA	55.0	40.9	57.9	57.8	797 bp	1.0	61.8
cemA_p2	GAGTCGACGAATGAGATGGG	TGCTTTGCTTATGTACAACTGC	55.0	40.9	57.9	57.8	798 bp	1.0	61.8
cemA_p3	CGACGAATGAGATGGGTTCA	GCTTTGCTTATGTACAACTGCA	50.0	40.9	57.7	57.8	793 bp	1.0	60.5
cemA_p4	CGACGAATGAGATGGGTTCA	TGCTTTGCTTATGTACAACTGC	50.0	40.9	57.7	57.8	794 bp	1.0	60.5
cemA_p5	GTCGACGAATGAGATGGGTT	GCTTTGCTTATGTACAACTGCA	50.0	40.9	57.7	57.8	795 bp	1.0	60.5