Hotspot

ccsA

SSC · 113878-114846 · 969 bp

Back to Ainsliaea

Pi 0.00103

MarkerSeek score 49.70

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 969
variable_sites: 1
pi: 0.00103
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GGCGGCATGTCATCTTCTAA	ACACCGTATTCCACTCTGTT	50.0	45.0	58.0	56.5	1129 bp	1.0	67.6
ccsA_p2	GGCGGCATGTCATCTTCTAA	ACACCGTATTCCACTCTGTTT	50.0	42.9	58.0	57.2	1129 bp	1.0	65.6
ccsA_p3	GGCGGCATGTCATCTTCTAA	ACCGTATTCCACTCTGTTTGT	50.0	42.9	58.0	57.2	1127 bp	1.0	65.6
ccsA_p4	GGCGGCATGTCATCTTCTAA	CACCGTATTCCACTCTGTTTG	50.0	47.6	58.0	56.9	1128 bp	1.0	63.0
ccsA_p5	GGCGGCATGTCATCTTCTAA	TACACCGTATTCCACTCTGT	50.0	45.0	58.0	55.6	1130 bp	1.0	60.6