Hotspot

accD-psaI

LSC · 58727-59428 · 702 bp

Back to Ainsliaea

Pi 0.00142

MarkerSeek score 59.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 1
pi: 0.00142
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AAAAAGCGTTCTGAGTGAGT	AAAGAAGCCATTGCGATTGC	40.0	45.0	55.5	58.0	840-847 bp	1.0	60.4
accD-psaI_p2	AAAAGCGTTCTGAGTGAGTT	AAAGAAGCCATTGCGATTGC	40.0	45.0	55.5	58.0	839-846 bp	1.0	60.4
accD-psaI_p3	TCAACTCCACACTTTCTTTCCT	AAAGAAGCCATTGCGATTGC	40.9	45.0	57.5	58.0	816-823 bp	1.0	60.1
accD-psaI_p4	AAAAAGCGTTCTGAGTGAGT	CTAAAGGCACCAAAACAGCG	40.0	50.0	55.5	57.9	803-810 bp	1.0	60.0
accD-psaI_p5	AAAAGCGTTCTGAGTGAGTT	CTAAAGGCACCAAAACAGCG	40.0	50.0	55.5	57.9	802-809 bp	1.0	60.0