Hotspot

atpB-rbcL

LSC · 54508-55269 · 762 bp

Back to Ainsliaea

Pi 0.00131

MarkerSeek score 59.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 1
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CTGGCGGAAAAGCTACATCT	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.0	58.0	923-930 bp	1.0	71.7
atpB-rbcL_p2	CTGGCGGAAAAGCTACATCT	ACCAGCTTTGAATCCAACACT	50.0	42.9	58.0	58.1	922-929 bp	1.0	71.4
atpB-rbcL_p3	GGAAACCCCAGAACCAGAAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	58.1	58.0	850-857 bp	1.0	71.2
atpB-rbcL_p4	ATGATTTGGACGATACGCCC	CACCAGCTTTGAATCCAACAC	50.0	47.6	58.1	58.0	891-898 bp	1.0	71.0
atpB-rbcL_p5	GGAAACCCCAGAACCAGAAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	58.1	58.1	849-856 bp	1.0	71.0