Hotspot

trnT-GGU-psbD

LSC · 31239-32485 · 1247 bp

Back to Ainsliaea

Pi 0.0016

MarkerSeek score 52.00

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1247
variable_sites: 2
pi: 0.0016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	ATCCGATACGATAAGGGGCT	GGACCAGCCTACAAAAACGA	50.0	50.0	57.8	58.1	1369-1370 bp	1.0	77.3
trnT-GGU-psbD_p2	CGTAAGCCCTCGGTTCAAAT	GGACCAGCCTACAAAAACGA	50.0	50.0	58.3	58.1	1387-1388 bp	1.0	77.1
trnT-GGU-psbD_p3	ATCCGATACGATAAGGGGCT	ACAAAAACGAAACGGTCCCT	50.0	45.0	57.8	58.2	1359-1360 bp	1.0	76.3
trnT-GGU-psbD_p4	CGTAAGCCCTCGGTTCAAAT	ACAAAAACGAAACGGTCCCT	50.0	45.0	58.3	58.2	1377-1378 bp	1.0	76.0
trnT-GGU-psbD_p5	TAAGCCCTCGGTTCAAATCC	GGACCAGCCTACAAAAACGA	50.0	50.0	57.6	58.1	1385-1386 bp	1.0	75.9