Hotspot

trnE-UUC-rpoB

LSC · 12059-12980 · 922 bp

Back to Ainsliaea

Pi 0.00108
MarkerSeek score 59.40
Primer available yes
Label trnE-UUC-rpoB

Feature Metadata

label_name
trnE-UUC-rpoB
feature_type
igs
region
LSC
length_bp
922
variable_sites
1
pi
0.00108
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-rpoB_p1 GAAGTCGTAACCACTGGACC AGTTCTTCTGTCAAACCCTGA 55.0 42.9 57.9 56.8 1029 bp 1.0 61.8
trnE-UUC-rpoB_p2 TAACCACTGGACCATAGGGG AGTTCTTCTGTCAAACCCTGA 55.0 42.9 58.1 56.8 1022 bp 1.0 61.5
trnE-UUC-rpoB_p3 AACCCTCGTTATCTCCGTGA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.2 56.8 1055 bp 1.0 61.1
trnE-UUC-rpoB_p4 ACCCTCGTTATCTCCGTGAA AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.2 56.8 1054 bp 1.0 61.1
trnE-UUC-rpoB_p5 TGAACCCTCGTTATCTCCGT AGTTCTTCTGTCAAACCCTGA 50.0 42.9 58.2 56.8 1057 bp 1.0 61.1