Hotspot

psbM-trnD-GUC

LSC · 10942-11601 · 660 bp

Back to Ainsliaea

Pi 0.00152

MarkerSeek score 60.10

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 660
variable_sites: 1
pi: 0.00152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGAACTAAAATGAACAGTGCAGT	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.6	58.3	774-777 bp	1.0	50.8
psbM-trnD-GUC_p2	AAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	40.0	50.0	54.5	58.3	768-771 bp	1.0	50.4
psbM-trnD-GUC_p3	ACTAAAATGAACAGTGCAGTAGC	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.4	58.3	771-774 bp	1.0	49.4
psbM-trnD-GUC_p4	AGGAACTAAAATGAACAGTGCAG	TTCAATAGGCAAGAGCACCG	39.1	50.0	57.3	58.3	775-778 bp	1.0	48.7
psbM-trnD-GUC_p5	AGGAACTAAAATGAACAGTGCA	TTCAATAGGCAAGAGCACCG	36.4	50.0	56.2	58.3	775-778 bp	1.0	47.9