Hotspot

psbK-psbI

LSC · 7789-8202 · 414 bp

Back to Ainsliaea

Pi 0.00242

MarkerSeek score 56.30

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 414
variable_sites: 1
pi: 0.00242
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	551 bp	1.0	72.2
psbK-psbI_p2	TATGCCAGTGATACCCCTGT	TCTTCACGTCCAGGATTACG	50.0	50.0	57.8	57.1	591 bp	1.0	71.1
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	548 bp	1.0	70.2
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	549 bp	1.0	70.2
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	TCTTCACGTCCAGGATTACG	45.0	50.0	58.3	57.1	550 bp	1.0	70.2