Hotspot

ycf53

SSC · 57955-58686 · 732 bp

Back to Ahnfeltia

Pi 0.489

MarkerSeek score 82.30

Primer available yes

Label ycf53

Feature Metadata

label_name: ycf53
feature_type: gene
region: SSC
length_bp: 732
variable_sites: 324
pi: 0.489
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf53_p1	TGGCTATTCAGTTCTCGAGG	ACAGAGGCCTTAATCACCCT	50.0	50.0	56.7	58.0	1383 bp	0.5	49.7
ycf53_p2	TGGCTATTCAGTTCTCGAGG	AGAGGCCTTAATCACCCTGT	50.0	50.0	56.7	58.0	1381 bp	0.5	49.7
ycf53_p3	GGCTATTCAGTTCTCGAGGT	ACAGAGGCCTTAATCACCCT	50.0	50.0	56.7	58.0	1382 bp	0.5	49.7
ycf53_p4	GGCTATTCAGTTCTCGAGGT	AGAGGCCTTAATCACCCTGT	50.0	50.0	56.7	58.0	1380 bp	0.5	49.7
ycf53_p5	TGGCTATTCAGTTCTCGAGG	CACAGAGGCCTTAATCACCC	50.0	55.0	56.7	58.0	1384 bp	0.5	49.6