Hotspot

rne

SSC · 51645-53093 · 1449 bp

Back to Ahnfeltia

Pi 0.483

MarkerSeek score 79.90

Primer available yes

Label rne

Feature Metadata

label_name: rne
feature_type: gene
region: SSC
length_bp: 1449
variable_sites: 572
pi: 0.483
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rne_p1	CTCTAGGTATCTCCGGCACA	ATTATGGGTTGAACCTGGGC	55.0	50.0	58.0	57.8	2810 bp	0.5	58.6
rne_p2	CTCTAGGTATCTCCGGCACA	GAGGTCAGATTGTGCTTGCT	55.0	50.0	58.0	58.2	2433 bp	0.5	58.4
rne_p3	CTCTAGGTATCTCCGGCACA	TGGAGGTCAGATTGTGCTTG	55.0	50.0	58.0	57.8	2435 bp	0.5	58.3
rne_p4	CCCAATTGGTCAAGGAAGCT	ATTATGGGTTGAACCTGGGC	50.0	50.0	58.1	57.8	2845 bp	0.5	58.2
rne_p5	CTCTAGTTGCGCCTTCTGTT	ATTATGGGTTGAACCTGGGC	50.0	50.0	57.9	57.8	2273 bp	0.5	58.0