Hotspot

psaF

SSC · 64743-65318 · 576 bp

Back to Ahnfeltia

Pi 0.499

MarkerSeek score 81.00

Primer available yes

Label psaF

Feature Metadata

label_name: psaF
feature_type: gene
region: SSC
length_bp: 576
variable_sites: 252
pi: 0.499
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaF_p1	TTCCTTGCACCTTCAGCATT	CTTTTGCCCAATCCTTTGCC	45.0	50.0	58.0	58.2	1730 bp	0.5	58.5
psaF_p2	TTCCTTGCACCTTCAGCATT	ACCCCATCAGGATCTGTCAT	45.0	50.0	58.0	57.8	1853 bp	0.5	58.4
psaF_p3	TTCCTTGCACCTTCAGCATT	CCCAGATGGTGGCATTGAAT	45.0	50.0	58.0	58.2	1684 bp	0.5	58.3
psaF_p4	TTCCTTGCACCTTCAGCATT	ATCCTTGTCGAGACTCAGGT	45.0	50.0	58.0	57.8	1494 bp	0.5	58.2
psaF_p5	TCGGAACACCCAAAGAGTTG	CTTTTGCCCAATCCTTTGCC	50.0	50.0	58.0	58.2	1867 bp	0.5	58.2