Hotspot

fabH

SSC · 62560-63573 · 1014 bp

Back to Ahnfeltia

Pi 0.447

MarkerSeek score 83.40

Primer available yes

Label fabH

Feature Metadata

label_name: fabH
feature_type: gene
region: SSC
length_bp: 1014
variable_sites: 428
pi: 0.447
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
fabH_p1	AATCCACAGTCCTTCAGGGA	AATGCTGAAGGTGCAAGGAA	50.0	45.0	58.0	58.0	2231 bp	0.5	59.6
fabH_p2	GGGAACACTGTGGACAAACT	AATGCTGAAGGTGCAAGGAA	50.0	45.0	57.9	58.0	2307 bp	0.5	59.6
fabH_p3	AATCCACAGTCCTTCAGGGA	CAACTCTTTGGGTGTTCCGA	50.0	50.0	58.0	58.0	2094 bp	0.5	59.3
fabH_p4	GGGAACACTGTGGACAAACT	CAACTCTTTGGGTGTTCCGA	50.0	50.0	57.9	58.0	2170 bp	0.5	59.3
fabH_p5	TAGCAACGCCGCTGTAATAG	AATGCTGAAGGTGCAAGGAA	50.0	45.0	58.2	58.0	1906 bp	0.5	58.8