Hotspot

carA

SSC · 58707-59858 · 1152 bp

Back to Ahnfeltia

Pi 0.435

MarkerSeek score 82.20

Primer available yes

Label carA

Feature Metadata

label_name: carA
feature_type: gene
region: SSC
length_bp: 1152
variable_sites: 451
pi: 0.435
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
carA_p1	GGGATAAAATCGGCTGGACA	GCATTTGGTGGTCGTTTAGC	50.0	50.0	57.7	58.0	1594 bp	0.5	57.1
carA_p2	TGGGATAAAATCGGCTGGAC	GCATTTGGTGGTCGTTTAGC	50.0	50.0	57.7	58.0	1595 bp	0.5	57.1
carA_p3	GGGATAAAATCGGCTGGACA	TCTCAGCTAATTGTTCGGCC	50.0	50.0	57.7	58.0	1743 bp	0.5	57.1
carA_p4	TGGGATAAAATCGGCTGGAC	TCTCAGCTAATTGTTCGGCC	50.0	50.0	57.7	58.0	1744 bp	0.5	57.1
carA_p5	GGGATAAAATCGGCTGGACA	CTCAGCTAATTGTTCGGCCT	50.0	50.0	57.7	58.0	1742 bp	0.5	57.0