Hotspot

apcD

SSC · 63932-64417 · 486 bp

Back to Ahnfeltia

Pi 0.457

MarkerSeek score 80.90

Primer available yes

Label apcD

Feature Metadata

label_name: apcD
feature_type: gene
region: SSC
length_bp: 486
variable_sites: 208
pi: 0.457
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
apcD_p1	TAAATCAGGAACGGCAGAGC	TGATGGCAGATGGACACATG	50.0	50.0	58.0	58.0	1500 bp	0.5	59.4
apcD_p2	TGTAGAAGCCAATGCAGCAA	TGATGGCAGATGGACACATG	45.0	50.0	58.1	58.0	1635 bp	0.5	58.9
apcD_p3	TTGTAGAAGCCAATGCAGCA	TGATGGCAGATGGACACATG	45.0	50.0	58.1	58.0	1636 bp	0.5	58.9
apcD_p4	TAAATCAGGAACGGCAGAGC	ACATGGCCATTAGCTGCTTT	50.0	45.0	58.0	58.1	1316 bp	0.5	58.7
apcD_p5	AGAAGCCAATGCAGCAAGAT	TGATGGCAGATGGACACATG	45.0	50.0	58.2	58.0	1632 bp	0.5	58.4