Hotspot

cpeB-cpeA

LSC · 11810-11906 · 97 bp

Back to Ahnfeltia

Pi 0.0515

MarkerSeek score 81.40

Primer available yes

Label cpeB-cpeA

Feature Metadata

label_name: cpeB-cpeA
feature_type: igs
region: LSC
length_bp: 97
variable_sites: 5
pi: 0.0515
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cpeB-cpeA_p1	GCTTCTCAACGCAAAATGGA	AAGGTCAGAACTGGAAGGGA	45.0	50.0	57.3	57.9	268 bp	1.0	74.9
cpeB-cpeA_p2	GCTTCTCAACGCAAAATGGA	AGGTCAGAACTGGAAGGGAA	45.0	50.0	57.3	57.9	267 bp	1.0	74.9
cpeB-cpeA_p3	AGCTTCTCAACGCAAAATGG	AAGGTCAGAACTGGAAGGGA	45.0	50.0	57.3	57.9	269 bp	1.0	74.8
cpeB-cpeA_p4	AGCTTCTCAACGCAAAATGG	AGGTCAGAACTGGAAGGGAA	45.0	50.0	57.3	57.9	268 bp	1.0	74.8
cpeB-cpeA_p5	GCTTCTCAACGCAAAATGGA	AGCACGTTGGATATTGCCTT	45.0	45.0	57.3	57.9	298 bp	1.0	74.7