Hotspot

ndhF

SSC · 102249-104468 · 2220 bp

Back to Agrostis

Pi 0.00616

MarkerSeek score 46.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 25
pi: 0.00616
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AACACCCATCAATGCATGGT	CGAAACAAGACAGCTGCTTC	45.0	50.0	58.1	57.7	2792-2808 bp	1.0	78.2
ndhF_p2	ACCCATCAATGCATGGTGTT	CGAAACAAGACAGCTGCTTC	45.0	50.0	58.1	57.7	2789-2805 bp	1.0	78.2
ndhF_p3	AACACCCATCAATGCATGGT	ACGAAACAAGACAGCTGCTT	45.0	45.0	58.1	58.3	2793-2809 bp	1.0	78.0
ndhF_p4	ACCCATCAATGCATGGTGTT	ACGAAACAAGACAGCTGCTT	45.0	45.0	58.1	58.3	2790-2806 bp	1.0	78.0
ndhF_p5	GCATGGTGTTCTTCGACTGA	CGAAACAAGACAGCTGCTTC	50.0	50.0	58.2	57.7	2779-2795 bp	1.0	77.0