Hotspot

rpl32-trnL-UAG

SSC · 105550-106211 · 662 bp

Back to Agrostis

Pi 0.0166

MarkerSeek score 58.40

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 662
variable_sites: 19
pi: 0.0166
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTTTTTCGGGGCAACAACAA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	732-738 bp	1.0	81.6
rpl32-trnL-UAG_p2	TTTTTCGGGGCAACAACAAC	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.0	57.9	731-737 bp	1.0	81.6
rpl32-trnL-UAG_p3	GTTTTTCGGGGCAACAACAA	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	725-731 bp	1.0	81.2
rpl32-trnL-UAG_p4	TTTTTCGGGGCAACAACAAC	AGCAGCGTGTCTACCAATTT	45.0	45.0	58.0	57.8	724-730 bp	1.0	81.2
rpl32-trnL-UAG_p5	TTTTTCGGGGCAACAACAAC	GCTTGAGCACTGCTTCCTAA	45.0	50.0	58.0	58.2	745-751 bp	1.0	81.1