Hotspot

rpl14-rpl16

LSC · 77514-77633 · 120 bp

Back to Agrostis

Pi 0.014

MarkerSeek score 61.80

Primer available yes

Label rpl14-rpl16

Feature Metadata

label_name: rpl14-rpl16
feature_type: igs
region: LSC
length_bp: 120
variable_sites: 3
pi: 0.014
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl14-rpl16_p1	AGCAGCTCCTATGACTCGAA	TAGCTGCCAGTAAAATGCCC	50.0	50.0	58.2	58.2	255-257 bp	1.0	77.8
rpl14-rpl16_p2	TACCAGCAGCTCCTATGACT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	57.5	58.2	259-261 bp	1.0	76.0
rpl14-rpl16_p3	AGCAGCTCCTATGACTCGAA	GCCAGTAAAATGCCCATACG	50.0	50.0	58.2	57.2	250-252 bp	1.0	73.6
rpl14-rpl16_p4	GCAGCTCCTATGACTCGAAT	TAGCTGCCAGTAAAATGCCC	50.0	50.0	57.2	58.2	254-256 bp	1.0	73.2
rpl14-rpl16_p5	ACCAGCAGCTCCTATGACTC	TAGCTGCCAGTAAAATGCCC	55.0	50.0	58.9	58.2	258-260 bp	1.0	72.6