Hotspot

petA-psbJ

LSC · 60659-61461 · 803 bp

Back to Agrostis

Pi 0.0118

MarkerSeek score 57.70

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 18
pi: 0.0118
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TGAAAAGGTTCAGTTGTACGA	CGGTTCATATTCTGGATTGGGT	38.1	45.5	55.4	58.2	871-878 bp	1.0	41.6
petA-psbJ_p2	TGAAAAGGTTCAGTTGTACGA	TCCTCTTTGGCTGATAGGTACT	38.1	45.5	55.4	58.0	937-944 bp	1.0	41.5
petA-psbJ_p3	TGAAAAGGTTCAGTTGTACGA	CCTCTTTGGCTGATAGGTACTG	38.1	50.0	55.4	57.9	936-943 bp	1.0	41.5
petA-psbJ_p4	ACAGTTTGAAAAGGTTCAGTTGT	TCCTCTTTGGCTGATAGGTACT	34.8	45.5	57.3	58.0	943-950 bp	1.0	41.5
petA-psbJ_p5	TGAAAAGGTTCAGTTGTACGA	TTCCTCTTTGGCTGATAGGT	38.1	45.0	55.4	55.8	938-945 bp	1.0	41.5