Hotspot

trnT-GGU-trnE-UUC

LSC · 14866-15398 · 533 bp

Back to Agrostis

Pi 0.0122

MarkerSeek score 63.10

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 533
variable_sites: 11
pi: 0.0122
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	AGCCCTTTTAACTCAGTGGT	AAATACTCCCTACCCCCAGG	45.0	55.0	56.7	57.8	686-691 bp	1.0	69.4
trnT-GGU-trnE-UUC_p2	AGGCATAAGTCATCGGTTCA	AAATACTCCCTACCCCCAGG	45.0	55.0	56.6	57.8	649-654 bp	1.0	69.3
trnT-GGU-trnE-UUC_p3	AGCCCTTTTAACTCAGTGGT	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.7	58.3	651-656 bp	1.0	69.1
trnT-GGU-trnE-UUC_p4	AGCCCTTTTAACTCAGTGGT	TGTCCTGAACCACTAGACGA	45.0	50.0	56.7	57.7	630-635 bp	1.0	69.0
trnT-GGU-trnE-UUC_p5	AGGCATAAGTCATCGGTTCA	GCTGCCTCCTTGAAAGAGAG	45.0	55.0	56.6	58.3	614-619 bp	1.0	69.0