Hotspot

trnG-UCC-trnT-GGU

LSC · 13590-14791 · 1202 bp

Back to Agrostis

Pi 0.0141
MarkerSeek score 59.20
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1202
variable_sites
31
pi
0.0141
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT ACCACTGAGTTAAAAGGGCT 38.1 45.0 55.5 56.7 1256-1270 bp 1.0 43.2
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT TGAACCGATGACTTATGCCT 38.1 45.0 55.5 56.6 1293-1307 bp 1.0 43.0
trnG-UCC-trnT-GGU_p3 GAACGAATCACACTTTTACCACT ACCACTGAGTTAAAAGGGCT 39.1 45.0 57.3 56.7 1258-1272 bp 1.0 42.3
trnG-UCC-trnT-GGU_p4 ACGAATCACACTTTTACCACT CCGATGACTTATGCCTTACCA 38.1 47.6 55.5 57.5 1289-1303 bp 1.0 42.2
trnG-UCC-trnT-GGU_p5 ACGAATCACACTTTTACCACT ACCGATGACTTATGCCTTACC 38.1 47.6 55.5 57.5 1290-1304 bp 1.0 42.2