Hotspot

trnS-GCU-psbD

LSC · 7677-8641 · 965 bp

Back to Agrostis

Pi 0.01

MarkerSeek score 65.80

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 965
variable_sites: 17
pi: 0.01
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GACAAAAACGAAACGGTCCC	45.0	50.0	58.0	57.9	1095-1104 bp	1.0	80.3
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1094-1103 bp	1.0	79.2
trnS-GCU-psbD_p3	ATTAGCAATCCGCCGCTTTA	CCGACAAAAACGAAACGGTC	45.0	50.0	58.0	58.3	1097-1106 bp	1.0	79.0
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	CGACAAAAACGAAACGGTCC	45.0	50.0	58.0	58.3	1096-1105 bp	1.0	79.0
trnS-GCU-psbD_p5	GAGCGGAAAGAGAGGGATTC	GACAAAAACGAAACGGTCCC	55.0	50.0	57.8	57.9	1146-1155 bp	1.0	78.7