Hotspot

rbcL

LSC · 54615-56048 · 1434 bp

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Pi 0

MarkerSeek score 35.30

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1434
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	TCAAGAGTTGTAGGGAGGGA	AAAGAAGAATTACTGCATTTCA	50.0	27.3	57.0	52.3	1549 bp	1.0	40.0
rbcL_p2	AGAGTTGTAGGGAGGGACTT	AAAGAAGAATTACTGCATTTCA	50.0	27.3	57.0	52.3	1546 bp	1.0	40.0
rbcL_p3	CAAGAGTTGTAGGGAGGGAC	AAAGAAGAATTACTGCATTTCA	55.0	27.3	56.9	52.3	1548 bp	1.0	40.0
rbcL_p4	TCAAGAGTTGTAGGGAGGGA	AGAAGAATTACTGCATTTCATAACT	50.0	28.0	57.0	55.1	1547 bp	1.0	40.0
rbcL_p5	TCAAGAGTTGTAGGGAGGGA	AAGAAGAATTACTGCATTTCAT	50.0	27.3	57.0	52.1	1548 bp	1.0	40.0