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rps11-rpl36

LSC · 74904-75096 · 193 bp

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Pi 0.00484

MarkerSeek score 52.50

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 2
pi: 0.00484
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	TCTACGTGCACTCTTCCGTA	GCGTGGACGAATTAGAGTCA	50.0	50.0	58.2	57.7	335-347 bp	1.0	79.6
rps11-rpl36_p2	CGTGCACTCTTCCGTAAACT	GCGTGGACGAATTAGAGTCA	50.0	50.0	58.2	57.7	331-343 bp	1.0	79.3
rps11-rpl36_p3	ATTCTACGTGCACTCTTCCG	GCGTGGACGAATTAGAGTCA	50.0	50.0	57.7	57.7	337-349 bp	1.0	78.8
rps11-rpl36_p4	TCTACGTGCACTCTTCCGTA	GGCGTGGACGAATTAGAGTC	50.0	55.0	58.2	58.4	336-348 bp	1.0	78.2
rps11-rpl36_p5	CGTGCACTCTTCCGTAAACT	GGCGTGGACGAATTAGAGTC	50.0	55.0	58.2	58.4	332-344 bp	1.0	78.0