Hotspot

ndhC-trnV-UAC

LSC · 49955-50862 · 908 bp

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Pi 0.000963
MarkerSeek score 50.80
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
908
variable_sites
2
pi
0.000963
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCCCTGAAATCGAAAATGCC GGTTCGAATCCGTATAGCCC 50.0 55.0 58.1 57.9 1010-1018 bp 1.0 79.6
ndhC-trnV-UAC_p2 GCCCTGAAATCGAAAATGCC CGGTTCGAATCCGTATAGCC 50.0 55.0 58.1 58.3 1011-1019 bp 1.0 77.8
ndhC-trnV-UAC_p3 CTCTGGTCCTTCACTAACCG GGTTCGAATCCGTATAGCCC 55.0 55.0 57.3 57.9 1039-1047 bp 1.0 74.9
ndhC-trnV-UAC_p4 TCTGGTCCTTCACTAACCGG GGTTCGAATCCGTATAGCCC 55.0 55.0 58.7 57.9 1038-1046 bp 1.0 74.3
ndhC-trnV-UAC_p5 TCACTAACCGGGGCTAAAAG GGTTCGAATCCGTATAGCCC 50.0 55.0 57.2 57.9 1029-1037 bp 1.0 74.0