Hotspot

psbM-petN

LSC · 16799-17364 · 566 bp

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Pi 0.000589

MarkerSeek score 46.00

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 566
variable_sites: 1
pi: 0.000589
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 46.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGCTACTGCACTGTTCATTCT	AGTATGGGGAAGGAGTGGAC	42.9	55.0	57.6	58.1	651-676 bp	1.0	70.2
psbM-petN_p2	TGCTACTGCACTGTTCATTCT	GTATGGGGAAGGAGTGGACT	42.9	55.0	57.6	58.1	650-675 bp	1.0	70.2
psbM-petN_p3	TGCTACTGCACTGTTCATTCT	TAGTAAGTCTCGCTTGGGCT	42.9	50.0	57.6	58.2	709-734 bp	1.0	69.8
psbM-petN_p4	TGCTACTGCACTGTTCATTCT	TATGGGGAAGGAGTGGACTC	42.9	55.0	57.6	57.8	649-674 bp	1.0	69.8
psbM-petN_p5	TGCTACTGCACTGTTCATTCT	GGGGAAGGAGTGGACTCTAG	42.9	60.0	57.6	58.2	646-671 bp	1.0	69.5