Hotspot

trnS-GCU-psbD

LSC · 7634-8607 · 974 bp

Back to Agropyron

Pi 0.000342

MarkerSeek score 46.70

Primer available yes

Label trnS-GCU-psbD

Feature Metadata

label_name: trnS-GCU-psbD
feature_type: igs
region: LSC
length_bp: 974
variable_sites: 1
pi: 0.000342
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psbD_p1	ATTAGCAATCCGCCGCTTTA	GAGCAATAGGCCAGACCATC	45.0	55.0	58.0	58.1	1127-1129 bp	1.0	79.0
trnS-GCU-psbD_p2	ATTAGCAATCCGCCGCTTTA	ACAAAAACGAAACGGTCCCT	45.0	45.0	58.0	58.2	1105-1107 bp	1.0	77.9
trnS-GCU-psbD_p3	GAGCGGAAAGAGAGGGATTC	GAGCAATAGGCCAGACCATC	55.0	55.0	57.8	58.1	1178-1180 bp	1.0	77.4
trnS-GCU-psbD_p4	ATTAGCAATCCGCCGCTTTA	CCCTTCGTAACCAGTCATCC	45.0	55.0	58.0	57.7	1089-1091 bp	1.0	77.4
trnS-GCU-psbD_p5	ATTAGCAATCCGCCGCTTTA	AGGCCAGACCATCCTACAAA	45.0	50.0	58.0	58.3	1120-1122 bp	1.0	77.2