Hotspot

rpl22-rps19

LSC · 85219-85275 · 57 bp

Back to Agriophyllum

Pi 0.0702

MarkerSeek score 71.70

Primer available yes

Label rpl22-rps19

Feature Metadata

label_name: rpl22-rps19
feature_type: igs
region: LSC
length_bp: 57
variable_sites: 4
pi: 0.0702
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl22-rps19_p1	CACCGGTACAGACGAAAGAC	ACGATAATAAGTCTCGTCGT	55.0	40.0	58.3	53.8	157 bp	1.0	46.0
rpl22-rps19_p2	CACCGGTACAGACGAAAGAC	AACGATAATAAGTCTCGTCGT	55.0	38.1	58.3	54.7	158 bp	1.0	44.9
rpl22-rps19_p3	CACCGGTACAGACGAAAGAC	ACGATAATAAGTCTCGTCGTT	55.0	38.1	58.3	54.7	157 bp	1.0	44.9
rpl22-rps19_p4	CACCGGTACAGACGAAAGAC	AAACGATAATAAGTCTCGTCGT	55.0	36.4	58.3	55.4	159 bp	1.0	43.1
rpl22-rps19_p5	CACCGGTACAGACGAAAGAC	AACGATAATAAGTCTCGTCGTT	55.0	36.4	58.3	55.4	158 bp	1.0	43.1