Hotspot

rbcL-accD

LSC · 57179-57794 · 616 bp

Back to Agriophyllum

Pi 0.0342
MarkerSeek score 68.10
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
616
variable_sites
19
pi
0.0342
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
68.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TTTGAATTCCCAGCAATGGA CATTTCCTGTCAGAATAAGCA 40.0 38.1 55.5 53.9 615-668 bp 1.0 46.4
rbcL-accD_p2 TTGAATTCCCAGCAATGGAT CATTTCCTGTCAGAATAAGCA 40.0 38.1 55.2 53.9 614-667 bp 1.0 46.4
rbcL-accD_p3 TTTGAATTCCCAGCAATGGA CATTTCCTGTCAGAATAAGCAT 40.0 36.4 55.5 54.5 615-668 bp 1.0 46.4
rbcL-accD_p4 ATTTGAATTCCCAGCAATGGA CATTTCCTGTCAGAATAAGCA 38.1 38.1 56.1 53.9 616-669 bp 1.0 46.4
rbcL-accD_p5 TTTGAATTCCCAGCAATGGAT CATTTCCTGTCAGAATAAGCA 38.1 38.1 56.1 53.9 615-668 bp 1.0 46.4