Hotspot

ndhC-trnV-UAC

LSC · 51193-51958 · 766 bp

Back to Agriophyllum

Pi 0.0275
MarkerSeek score 72.10
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
766
variable_sites
21
pi
0.0275
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 CCTTTGCTAACCGGAGCTAA AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.6 57.8 909-958 bp 1.0 77.2
ndhC-trnV-UAC_p2 TCCTTTGCTAACCGGAGCTA AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.4 57.8 910-959 bp 1.0 76.9
ndhC-trnV-UAC_p3 TTCTCGGGTCCTTTGCTAAC AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.5 57.8 918-967 bp 1.0 76.6
ndhC-trnV-UAC_p4 CCTTTGCTAACCGGAGCTAA GAAGGTCTACGGTTCGAGTC 50.0 55.0 57.6 57.5 908-957 bp 1.0 74.5
ndhC-trnV-UAC_p5 TCCTTTGCTAACCGGAGCTA GAAGGTCTACGGTTCGAGTC 50.0 55.0 58.4 57.5 909-958 bp 1.0 74.2