Hotspot

rpoC2

LSC · 16133-20266 · 4134 bp

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Pi 0.00126

MarkerSeek score 35.60

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4134
variable_sites: 15
pi: 0.00126
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 35.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	AATTAAGGACTCATTCGCTC	GGATTTGTGCGCCTTTCAAG	40.0	50.0	53.1	58.3	200 bp	1.0	40.8
rpoC2_p2	AAATTAAGGACTCATTCGCTC	GGATTTGTGCGCCTTTCAAG	38.1	50.0	54.0	58.3	201 bp	1.0	40.8
rpoC2_p3	AATTAAGGACTCATTCGCTC	AAAGGATTTGTGCGCCTTTC	40.0	45.0	53.1	57.6	203 bp	1.0	40.8
rpoC2_p4	AAATTAAGGACTCATTCGCTC	AAAGGATTTGTGCGCCTTTC	38.1	45.0	54.0	57.6	204 bp	1.0	40.8
rpoC2_p5	AAAATTAAGGACTCATTCGCTC	GGATTTGTGCGCCTTTCAAG	36.4	50.0	54.8	58.3	202 bp	1.0	40.8