Hotspot

rpl16

LSC · 81542-82915 · 1374 bp

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Pi 0.00555

MarkerSeek score 44.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1374
variable_sites: 17
pi: 0.00555
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 44.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	TTAATTCCCGAGGGATTGCG	GTCGGATCGACGGAAAAGAA	50.0	50.0	58.0	58.0	2131 bp	0.166667	46.3
rpl16_p2	TTAATTCCCGAGGGATTGCG	GTTCCTACGCAGTTCGAACT	50.0	50.0	58.0	57.9	2041 bp	0.166667	45.9
rpl16_p3	TTAATTCCCGAGGGATTGCG	GTCGAGGGAATTGCACGTAT	50.0	50.0	58.0	58.1	2459 bp	0.166667	45.8
rpl16_p4	TTAATTCCCGAGGGATTGCG	CTGAGCAGGCAGGTACAAAA	50.0	50.0	58.0	58.1	2173 bp	0.166667	45.5
rpl16_p5	TTAATTCCCGAGGGATTGCG	TGAGCAGGCAGGTACAAAAG	50.0	50.0	58.0	58.1	2172 bp	0.166667	45.5