Hotspot

rpl32-trnL-UAG

SSC · 114087-114887 · 801 bp

Back to Agrimonia

Pi 0.00932

MarkerSeek score 61.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 801
variable_sites: 19
pi: 0.00932
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GTAAAGGGTATTGGGCAGCA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	887-935 bp	1.0	83.6
rpl32-trnL-UAG_p2	GGGCAGCATTGAAAGCTTTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.8	57.9	875-923 bp	1.0	83.3
rpl32-trnL-UAG_p3	GTAAAGGGTATTGGGCAGCA	AGCAGCGTGTCTACCAATTT	50.0	45.0	57.9	57.8	880-928 bp	1.0	83.1
rpl32-trnL-UAG_p4	GTAAAGGGTATTGGGCAGCA	CCTAAGAGCAGCGTGTCTAC	50.0	55.0	57.9	57.8	886-934 bp	1.0	83.0
rpl32-trnL-UAG_p5	GTAAAGGGTATTGGGCAGCA	CTAAGAGCAGCGTGTCTACC	50.0	55.0	57.9	57.8	885-933 bp	1.0	83.0