Hotspot

rps19-rpl2

IRb · 84627-84682 · 56 bp

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Pi 0.0179

MarkerSeek score 60.00

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 56
variable_sites: 3
pi: 0.0179
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	ATGGTAGATGCTCGAGACCA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	57.9	58.1	200-205 bp	1.0	80.6
rps19-rpl2_p2	ATGGTAGATGCTCGAGACCA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	57.9	58.1	292-297 bp	1.0	80.3
rps19-rpl2_p3	TGGTAGATGCTCGAGACCAA	TTGATTCTTCGTCGCCGTAG	50.0	50.0	58.2	58.1	199-204 bp	1.0	80.0
rps19-rpl2_p4	TGGTAGATGCTCGAGACCAA	GGGCCCCAATTGGTAGAAAA	50.0	50.0	58.2	58.1	291-296 bp	1.0	79.7
rps19-rpl2_p5	ATGGTAGATGCTCGAGACCA	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.9	57.8	258-263 bp	1.0	79.2