Hotspot

rpl20-rps12

LSC · 69097-69901 · 805 bp

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Pi 0.00381

MarkerSeek score 55.60

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 805
variable_sites: 8
pi: 0.00381
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TTGTTCTACGTCTTCGAGCG	GAAATCCCCTGCTCTTGGAG	50.0	55.0	58.0	58.0	908-909 bp	1.0	80.1
rpl20-rps12_p2	TCGAGCGATATACCCTCGTT	GAAATCCCCTGCTCTTGGAG	50.0	55.0	58.1	58.0	895-896 bp	1.0	79.5
rpl20-rps12_p3	TTCGAGCGATATACCCTCGT	GAAATCCCCTGCTCTTGGAG	50.0	55.0	58.1	58.0	896-897 bp	1.0	79.5
rpl20-rps12_p4	CGTCTTCGAGCGATATACCC	GAAATCCCCTGCTCTTGGAG	55.0	55.0	57.8	58.0	900-901 bp	1.0	78.4
rpl20-rps12_p5	GTTCTACGTCTTCGAGCGAT	GAAATCCCCTGCTCTTGGAG	50.0	55.0	57.5	58.0	906-907 bp	1.0	76.8