Hotspot

trnP-UGG-psaJ

LSC · 66714-67168 · 455 bp

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Pi 0.00593
MarkerSeek score 57.00
Primer available yes
Label trnP-UGG-psaJ

Feature Metadata

label_name
trnP-UGG-psaJ
feature_type
igs
region
LSC
length_bp
455
variable_sites
7
pi
0.00593
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.94
markerseek_score
57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP-UGG-psaJ_p1 GGATGACAGGATTCGAACCC AACCTGCTAAAGACCCGAAC 55.0 50.0 58.0 57.8 563-599 bp 1.0 83.2
trnP-UGG-psaJ_p2 GGGATGACAGGATTCGAACC AACCTGCTAAAGACCCGAAC 55.0 50.0 58.0 57.8 564-600 bp 1.0 83.2
trnP-UGG-psaJ_p3 GGATGACAGGATTCGAACCC AGTACTTACTACCGGTGCCA 55.0 50.0 58.0 57.8 538-574 bp 1.0 82.9
trnP-UGG-psaJ_p4 GGGATGACAGGATTCGAACC AGTACTTACTACCGGTGCCA 55.0 50.0 58.0 57.8 539-575 bp 1.0 82.9
trnP-UGG-psaJ_p5 AAAACAAACGCGCTACCAAG AACCTGCTAAAGACCCGAAC 45.0 50.0 57.9 57.8 527-563 bp 1.0 82.7