Hotspot

ndhC-trnV-UAC

LSC · 51115-51766 · 652 bp

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Pi 0.0119

MarkerSeek score 49.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 652
variable_sites: 21
pi: 0.0119
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CCCCATTGGTTCTATTCCCG	TAAATCAGAGCACGTGGAGC	55.0	50.0	58.0	58.0	995-1003 bp	1.0	82.2
ndhC-trnV-UAC_p2	CCCCATTGGTTCTATTCCCG	ACGTGGAGCCATTTTTCTGT	55.0	45.0	58.0	58.0	984-992 bp	1.0	82.1
ndhC-trnV-UAC_p3	AGAGAAGCGGCCATAAACTG	TAAATCAGAGCACGTGGAGC	50.0	50.0	58.0	58.0	1364-1372 bp	1.0	81.4
ndhC-trnV-UAC_p4	AGAGAAGCGGCCATAAACTG	ACGTGGAGCCATTTTTCTGT	50.0	45.0	58.0	58.0	1353-1361 bp	1.0	81.4
ndhC-trnV-UAC_p5	CCTGTCTCGGACTAGATCGT	TAAATCAGAGCACGTGGAGC	55.0	50.0	58.0	58.0	1463-1471 bp	1.0	81.2