Hotspot

psbM-trnD-GUC

LSC · 29760-30760 · 1001 bp

Back to Agrimonia

Pi 0.003
MarkerSeek score 58.20
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1001
variable_sites
8
pi
0.003
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 TCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.6 58.5 1076-1166 bp 1.0 77.3
psbM-trnD-GUC_p2 AATCACCTTGGCTGACTGTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1078-1168 bp 1.0 75.4
psbM-trnD-GUC_p3 ATCACCTTGGCTGACTGTTT TTCAATTGGTCAGAGCACCG 45.0 50.0 57.3 58.5 1077-1167 bp 1.0 75.4
psbM-trnD-GUC_p4 TCACCTTGGCTGACTGTTTT GTTCAATTGGTCAGAGCACC 45.0 50.0 57.6 57.0 1077-1167 bp 1.0 73.1
psbM-trnD-GUC_p5 ATCACCTTGGCTGACTGTTTT TTCAATTGGTCAGAGCACCG 42.9 50.0 58.1 58.5 1077-1167 bp 1.0 72.3