Hotspot

rps2-rpoC2

LSC · 15913-16132 · 220 bp

Back to Agrimonia

Pi 0.0276

MarkerSeek score 58.50

Primer available yes

Label rps2-rpoC2

Feature Metadata

label_name: rps2-rpoC2
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 14
pi: 0.0276
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2-rpoC2_p1	CCTGCTTCCATCATCTCTTCC	TTTTTCGGATTTAATGATTCCT	52.4	27.3	58.2	52.1	285-294 bp	1.0	46.3
rps2-rpoC2_p2	AGGTGCCATCCTAGGATTCC	TTTTTCGGATTTAATGATTCCT	55.0	27.3	58.6	52.1	334-343 bp	1.0	45.4
rps2-rpoC2_p3	GGTGCCATCCTAGGATTCCA	TTTTTCGGATTTAATGATTCCT	55.0	27.3	58.9	52.1	333-342 bp	1.0	45.4
rps2-rpoC2_p4	AGGTGCCATCCTAGGATTCC	ACATGATACATCAAAACAATCTT	55.0	26.1	58.6	52.7	356-365 bp	1.0	45.1
rps2-rpoC2_p5	AGGTGCCATCCTAGGATTCC	ACATGATACATCAAAACAATCTTT	55.0	25.0	58.6	53.5	356-365 bp	1.0	45.1