Hotspot

trnR-UCU-atpA

LSC · 10069-10670 · 602 bp

Back to Agrimonia

Pi 0.0202

MarkerSeek score 75.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 602
variable_sites: 27
pi: 0.0202
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	AATTGGAGTGAAAGGCGTCC	CAGGACCAGATGGAACGTTT	50.0	50.0	58.5	57.8	661-730 bp	1.0	81.9
trnR-UCU-atpA_p2	AGTGAAAGGCGTCCATTGTC	CAGGACCAGATGGAACGTTT	50.0	50.0	58.5	57.8	655-724 bp	1.0	81.9
trnR-UCU-atpA_p3	GAGTGAAAGGCGTCCATTGT	CAGGACCAGATGGAACGTTT	50.0	50.0	58.5	57.8	656-725 bp	1.0	81.9
trnR-UCU-atpA_p4	GTGAAAGGCGTCCATTGTCT	CAGGACCAGATGGAACGTTT	50.0	50.0	58.5	57.8	654-723 bp	1.0	81.9
trnR-UCU-atpA_p5	AATTGGAGTGAAAGGCGTCC	ACCGCAGTTCCAAGAAATCA	50.0	45.0	58.5	57.7	737-806 bp	1.0	80.9