Hotspot

rps16-trnQ-UUG

LSC · 6236-7160 · 925 bp

Back to Agrimonia

Pi 0.00681
MarkerSeek score 55.60
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
925
variable_sites
17
pi
0.00681
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.99
markerseek_score
55.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 988-1014 bp 1.0 80.9
rps16-trnQ-UUG_p2 TCCACAGACGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.1 58.1 1010-1036 bp 1.0 80.8
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 999-1025 bp 1.0 80.4
rps16-trnQ-UUG_p4 CTCCACAGACGATCATGTCC GAGGTTCGAATCCTTCCGTC 55.0 55.0 57.8 58.1 1011-1037 bp 1.0 80.3
rps16-trnQ-UUG_p5 CCACAGACGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.3 58.1 1009-1035 bp 1.0 76.5