Hotspot

rps15-ycf1

SSC · 132922-133613 · 692 bp

Back to Aglaonema

Pi 0.0261

MarkerSeek score 62.30

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 692
variable_sites: 16
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.89
markerseek_score: 62.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCAACGCGTTACATAAGG	TGAACCGGTTCGTCTCTCTA	50.0	50.0	58.0	57.8	1813-1881 bp	1.0	82.8
rps15-ycf1_p2	CAGACCCGCTGTTTTCTTCT	TGAACCGGTTCGTCTCTCTA	50.0	50.0	58.1	57.8	1282-1348 bp	1.0	82.8
rps15-ycf1_p3	ATCAGTCGAAGAACACCGTG	TGAACCGGTTCGTCTCTCTA	50.0	50.0	57.9	57.8	1687-1755 bp	1.0	82.7
rps15-ycf1_p4	TCTTGTAGCCGGTACAGTCA	TGAACCGGTTCGTCTCTCTA	50.0	50.0	58.1	57.8	1622-1690 bp	1.0	82.7
rps15-ycf1_p5	TTCCCAATTGTTCTGGTGCA	TGAACCGGTTCGTCTCTCTA	45.0	50.0	57.9	57.8	1872-1940 bp	1.0	82.1