Hotspot

psaC-ndhE

SSC · 125677-126845 · 1169 bp

Back to Aglaonema

Pi 0.0191

MarkerSeek score 66.30

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 1169
variable_sites: 22
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CGGCCAAGGTATCATTTCCA	AGCGGCTATTGGTCTAGCTA	50.0	50.0	57.9	58.0	1353-1361 bp	1.0	82.0
psaC-ndhE_p2	TGCTTAGCTTTACATCCCGG	AGCGGCTATTGGTCTAGCTA	50.0	50.0	57.7	58.0	1370-1378 bp	1.0	79.8
psaC-ndhE_p3	GGCCCGTACACATTGAGTAC	AGCGGCTATTGGTCTAGCTA	55.0	50.0	58.4	58.0	1317-1325 bp	1.0	79.6
psaC-ndhE_p4	GCCCGTACACATTGAGTACA	AGCGGCTATTGGTCTAGCTA	50.0	50.0	57.6	58.0	1316-1324 bp	1.0	79.5
psaC-ndhE_p5	CTTAGCTTTACATCCCGGCC	AGCGGCTATTGGTCTAGCTA	55.0	50.0	58.4	58.0	1368-1376 bp	1.0	79.1